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This research studied the effect of honey, coffee and vitamin E on blood glucose level in a non-diabetic experimental design. A total of 30 Wistar rats weighing 200-220 grams were used. The experimental period lasted for 56 days. Blood glucose test was carried out on days 0, 18, 37 and 56. Data derived from the experiment was presented after mean comparisons and percentage change evaluation. The result showed that honey and honey co-administered coffee or vitamin E time-dependently reduced blood glucose level significantly. Vitamin E alone did not have any significant effect on blood glucose, but coffee alone time-dependently increased blood glucose level significantly. From this study, honey may be effective in regulating blood glucose level, vitamin E, except co-administered honey, has no significant effect on blood glucose and coffee alone may cause a significant increase in blood glucose level, except if it is co-administered honey.