Main Article Content
Aim: To evaluate the effects of Pleurotus ostreatus on the lipid profile and atherogenic indices in Hyperlipidemic rats.
Study Design, Place and Duration of Study: This case-control study was done for 60 days between March and April, 2017 at the department of Medical Laboratory Science and Department of Chemical Pathology, Babcock University, Ogun State, Nigeria.
Methodology: Thirty male wistar rats weighing 117-130 g were divided randomly into 3 groups: Normolipidemic (NL) rats (fed with standard rodent chow), Hyperlipidemic (HL) rats (fed with standard rodent chow + duck yolk and reused oil), Hyperlipidemic Treated (HL+T) rats (fed with standard rodent chow + duck yolk and reused oil + 5% Pleurotus ostreatus powder).
Changes in the animal body weights were measured in this study. Serum was obtained from fasting blood samples for the standard biochemical analyses of total cholesterol (TC), triglycerides (TG), High density lipoprotein cholesterol (HDL-C), creatinine, urea, Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST). Low density lipoprotein (LDL), very low density lipoprotein (VLDL), TC/HDL, LDL/HDL and Log (TG/HDL) ratios were calculated.
Results: The HL+T rats compared to HL rats had significantly reduced body weight, TC, TG, LDL, VLDL, TC/HDL, LDL/HDL and Log(TG/HDL) by 19.59%, 14.38%, 15.82%, 25.52%, 15.83%, 28.89%, 20.24% and 27.27% respectively (p ≤ 0.05) but recorded no significant change in HDL-C (p > 0.05). Creatinine, urea, AST and ALT did not show any significant change in HL rats and HL+T rats (p > 0.05).
Conclusion: Treatment of hyperlipidemic male wistar rats with Pleurotus ostreatus reduced body weight, lipid levels (TC, TG, LDL, VLDL) and atherogenic indices (TC/HDL, LDL/HDL, Log (TG/HDL)) and appeared to have no detrimental effects on the liver and kidneys. These findings may provide insights and scientific basis for the promotion of the use of Pleurotus ostreatus in controlling hyperlipidemia and associated complications.
Tariq M, Rajab A. Comparative study for Atherogenic Index of Plasma (AIP) in patient with type1 Diabetes Mellitus, type2 Diabetes Mellitus, Betathalassemia and Hypothyroidism. Internation Journal of Chemistry Research. 2012;2:1-9.
Yokosuka T, Sasaki EJ, Okamoto CS, Sei Y. The protective role of chinese prescription Kangen-karyu extract on Diet-Induced Hypercholesterolemia in Rats. Biological and Pharmaceutical Bulletin. 2006;29(4):760-765.
Kalmis E, Nuri A, Hasan Y, Fatin K. Feasibility of using olive mill effluent as a wetting agent during the cultivation of oyster mushroom, Pleurotus ostreatus on wheat straw. Bioresource Technoogy. 2008;99:164-169.
Alam N, Yoon KN, Lee TS, Lee UY. Hypolipidemic activities of dietary Pleurotus ostreatus in hyper-cholesterolemic rats. Mycobiology. 2011; 39(1):45-51.
Sa’adah NN, Purwani KI, Nurhayati AP, Ashuri NM. Analysis of lipid profile and atherogenic index in hyperlipidemic rat (Rattus norvegicus Berkenhout, 1769) that given the methanolic extract of Parijoto (Medinilla speciosa). AIP Conference Proceedings. 2017;1854(1).
Wang D, Sakoda AK, Suzuki M. Biological efficiency and nutritional values of Pleurotous ostreatus cultivated on spent beer grain. Bioresource Technology. 2011; 78:93-300.
Buccolo G, David, H. Quantitative determination of serum Triglycerides by use of enzymes. Clinical Chemistry. 1973; 19(5):476–482.
Allain CC, Poon LS, Chan CS, Richmond W, Fu PC. Enzymatic determination of total serum cholesterol. Clinical Chemistry. 1974;20(4):470–475.
Burstein M, Scholnick HR, Morfin R. Rapid method for the isolation of lipoproteins from human serum by precipitation with polyanions. Scandavian Journal Clinical and Laboratory Investigations. 1980;40: 583-595.
Burtis CA, Ashwood ER. Teitz fundamentals of clinical chemistry. New Delhi, India: Reed Elsevier India Private Limited. 2006;348–488.
Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clinical Chemistry. 1990; 18:499-502.
Dobiášová M, Frohlich J, Šedová M, Cheung, MC, Brown BG. Cholesterol esterification and atherogenic index of plasma correlate with lipoprotein size and findings on coronary angiography. Journal of Lipid Research. 2011;52(3): 566–571.
Bobek P, Hromadova M, Ozdin L. Oyster mushroom (Pleurotus ostreatus) reduces the activity of 3-hydroxy-3- methyl-glutaryl coA reductase in rat liver microsomes. Experientia. 1995;51:589-591.
Nworu CS, Ihim, SA, Okoye FB, Esimone CO, Adikwu MU, Akah PA. Immunomodulatory and immunorestorative activities of β-d-glucan-rich extract and polysaccharide fraction of mushroom, Pleurutus tuberregium. Pharmaciticaul Biology. 2015;53(11):1555-1566.
Kim SW, Park SK, Kang SI, Kang HC, Oh HJ, Bae CY, et al. Hypocholesterolemic property of Yucca schidigera and Quillajasaponaria extracts in human body. Archives of Pharmaceutical Research. 2003;26:1042–1046.
Schena FP, Gesualdo L. Pathogenetic mechanisms of diabetic nephropathy. Journal of American Society of Nephrology. 2005;16(3):30-33.