Protective Role of Rockect Seed (Eruca sativa) Extract against Monosodium Glutamate-induced Hepato-renal Toxicity in Male Rats

Main Article Content

Ehab Tousson
Afaf El-Atrash
Yosra Karson

Abstract

Background and Objective: Monosodium glutamate (MSG) is identified as an Accent that is used in the food industry as a flavour enhancer with an umami taste that intensifies the meaty, savoury flavour of food. The present study aimed at evaluating the protective and ameliorative role of rocket seeds extract against monosodium glutamate-induced hepatic renal toxicity and oxidative stress in the male rat.

Materials and Methods: A total of 60 male adult albino rats were equally divided into six groups (G1, Control; G2, rocket seeds (RS); G3, ACCENT or MSG; G4, Co- treated (RS+MSG); G5, Post- treated (MSG+RS); G6, Self-treated MSG). 

Results: Current results revealed that; a significant increase in serum ALT, AST, ALP, AFP, Urea, Creatinine, potassium ions, chloride ions, cholesterol, triglyceride, HDL, and LDL levels in MSG as compared to control and RS groups. In contrast; a significant decrease in serum albumin, total proteins, catalase, GSH and SOD in liver and kidney homogenates in MSG as compared to control and RS groups. Co- or post-treatment of MSG with rocket seeds improved this change in liver and kidney functions, with best results for co-treatment than post and self-treatment.

Conclusion: These findings suggested that the misuse of monosodium glutamate may contribute to continuous hepatic and renal damage. This shows that the desired dose of monosodium glutamate can safely be used with grapes seed in improving hepatic and renal damage in monosodium glutamate in young rats.

Keywords:
Monosodium glutamate, ACCENT, rocket seeds, liver and kidney

Article Details

How to Cite
Tousson, E., El-Atrash, A., & Karson, Y. (2020). Protective Role of Rockect Seed (Eruca sativa) Extract against Monosodium Glutamate-induced Hepato-renal Toxicity in Male Rats. Asian Journal of Research in Medical and Pharmaceutical Sciences, 8(3-4), 1-10. https://doi.org/10.9734/ajrimps/2019/v8i3-430136
Section
Original Research Article

References

Simon RA, Allergic. Asthmatic reactions to food additives; 2015.
Available:http://www.uptodate.com/home.

Dixit SG, Rani P, Anand A, Khatri K, Chauhan R, Bharihoke V. To study the effect of monosodium glutamate on histomorphometry of cortex of kidney in adult albino rats. Ren fails. 2014;36(2): 266–270.

Löliger J. Function and importance of glutamate for savory foods. Journal of Nutrition. 2000;130:915-920.

Ikeda K. "New seasonings". Chemical Senses. 2002;27(9):847–9.

Leung A, Foster S. Encyclopedia of common natural ingredient used in food. Drugs and cosmetics, New york, wiley. 1996;373-375.

Walker R, Lupien JR. The safety evaluation of monosodium glutamate, Journal of Nutrition. 2000;130(4s suppl): 1049s-52s.

Eweka O. histological studies of the effects of monosodium glutamate on the kidney of adult wistar rats. The Internet Journal of Health. 2007;6(2).

Tousson E, Beltagy D, Gazia M, Al-Behbehani B. Expressions of p53 and cd68 in mouse liver with schistosoma mansoni infection and the protective role of silymarin. Toxicology and Industrial Health. 2013;29(8):761-70.

Elmasry TA, Al-Shaalan NH, Tousson E, El-Morshedy K, Al-Ghadeer A. Star anise extracts modulation of reproductive parameters, fertility potential and dna fragmentation induced by growth promoter equigan in rat testes. Brazilian Journal of Pharmaceutical Sciences. 2018;54(1): e17261.

Oyouni AA, Saggu S, Tousson E, Rehman H. Immunosuppressant drug tacrolimus induced mitochondrial nephrotoxicity, modified pcna and bcl-2 expression attenuated by ocimum basilicum l. In cd1 mice. Toxicology Reports. 2018;5:687-94.

Oyouni A, Saggu S, Tousson E, Mohan A, Farasani A. Mitochondrial nephrotoxicity induced by tacrolimus (fk-506) and modulatory effects of bacopa monnieri (farafakh) of tabuk region. Pharmacognosy Research. 2019;11(1):20.

Eldaim M, Tousson E, El Sayed IE, El Ae, Elsharkawy HN. Grape seeds proanthocyanidin extract ameliorates ehrlich solid tumor induced renal tissue and DNA damage in mice. Biomedicine & Pharmacotherapy. 2019;115:108908.

Abd Eldaim M, Tousson E, El Sayed I, Awd WM. Ameliorative effects of saussurea lappa root aqueous extract against ethephon‐induced reproductive toxicity in male rats. Environmental Toxicology. 2019;34(2):150-9.

Aldubayan M, Elgharabawy R, Ahmed A, Tousson E. Antineoplastic activity and curative role of avenanthramides against the growth of ehrlich solid tumors in mice. Oxidative Medicine and Cellular Longevity; 2019.

Moustafa A, Ali E, Moselhey S, Tousson E, El-Said K. Effect of coriander on thioacetamide-induced hepatotoxicity in rats. Toxicology and Industrial Health. 2014;30(7):621-9.

Saggu S, Sakeran M, Zidan N, Tousson E, Mohan A, Rehman H. Ameliorating effect of chicory (chichorium inty-bus l.) Fruit extract against 4-tert-octylphenol induced liverinjury and oxidative stress in male rats. Food and chemicaltoxicology. 2014; 72:138–146.

El-Masry T, Al-Shaalan N, Tousson E, El-Morshedy K, Al-Ghadeer A. P53 expression in response to equigan induced testicular injury and oxidative stress in male rat and the possible prophylactic effect of star anise extracts. Annual Research & Review in Biology. 2017;14(1):1-8.

Barillari J, Canistro D, Paolini M, Ferroni F, Pedulli G, Iori R, Valgimigli L. Direct antioxidant activity of purified glucoerucin, the dietary secondary metabolite contained in rocket (eruca sativa mill.) Seeds and sprouts. Journal of agricultural and Food Chemistry. 2005;53(7):2475-2482.

Lazzeri L, Errani M, Leoni O, Venturi G, Eruca Sativa Spp.Oleifera. A New Non-food Crops. 2004;20:67-73.

Chen Y, Ma J, Wang F, Hu J, Cui A, Wei C, Yang Q, Li F. Amygdalin induces apoptosis in human cervical cancer cell line hela cells. Immunopharmacology and Immunotoxicology. 2013;35(1):43-51.

Talalay P, Fahey JW. Antioxidant constituents of radish sporut (kaiware-daikon), (raphanus sativus l.). J. Agric. Food Chem. 2001;51:8061–6.

Bradley P. Reproduction et insemination artificielle en cuniculture. Anim. Reprod. Sci. 1992;70:139–49.

Leung A, Foster S. Method for the measurement of antioxidant activity in human fluids. J. Clin. Pathol. 1996;54: 356–61.

Kim S, Jin S, Ishii G. Gastroprotective activity of nigella sativa l oil and its constituent, thymoquinone against acute alcohol-induced gastric mucosal injury in rats. World j. Gastroenterol. 2004;11: 6662–6.

Abdo M, Zeinab. Effect of feeding different levels of herbal feed additives on broiler performance in relation to some metabolic function. Egypt Poult. Sci. 2003;15:111–39.

Bendich A. Direct antioxidant activity of purified glucoerucin, the dietary secondary metabolite contained in rocket (eruca sativa mill) seeds and sprouts. J. Agric. Food Chem. 1989;6:2475–82.

Bolkiny Y, Tousson E, El-Atrsh, A, Akela M, Farg, E. Costus. Root extract alleviates blood biochemical derangements of experimentally-induced hypo-and hyperthyroidism in mice. Annual Research & Review in Biology. 2019;1-10.

Al-Rasheed N, El-Masry T, Tousson E, Hassan H, Al-Ghadeer A. Hepatic protective effect of grape seed proanthocyanidin extract against gleevec-induced apoptosis, liver injury and ki67 alterations in rats. Brazilian Journal of Pharmaceutical Sciences. 2018 54(2).

Basuony M, Hafez E, Tousson E, Massoud A, Elsomkhraty S, Eldakamawy S. Beneficial role of panax ginseng root aqueous extract against cisplatin induced blood toxicity in rats. Am J Biol Chem. 2015;3(1):1-7.

Tousson E, Hafez E, Zaki S, Gad A. The cardioprotec-tive effects of l-carnitine on rat cardiac injury, apoptosis, andoxidative stress caused by amethopterin, Environmental Science and Pollution Research International. 2016;23(20): 20600–20608.

Tousson E, El‐Atrsh A, Mansour M, Abdallah A. Modulatory effects of Saussurea lappa root aqueous extract against ethephon‐induced kidney toxicity in male rats. Environmental Toxicology.‏ 2019;34(12):1277-1284.
DOI:10.1002/tox.22828

Salama AF, Tousson E, Shalaby KA, Hussien HT. Protective effect of curcumin on chloroform as by-product of water chlorination induced cardiotoxicity. Biomedicine & Preventive Nutrition. 2014; 4(2):225-230.

Salama AF, Kasem SM, Tousson E, Elsisy MK. Protective role of L-carnitine and vitamin E on the testis of atherosclerotic rats. Toxicology and Industrial Health. 2015;31(5):467-74.

Oscar O, Ebenezer O, Godwin E, Agwu I, Ando O. Godffery. Effect of vitamin e on monosodium gluta-mate induced hepatotoxicity and oxidative stress in rats. Indian Journal of Biophysics. 2006;43:20–24.

Onyema O, Farombi E, Emerole G, Ukoha,and A. Onyeze O. Effect of vitamin e on monosodiumglutamate induced hepatotoxicity and oxidative stress in rats. Indian Journal of Biochemistry & Bio-Physics. 2006;4(3):20–24

Almakhatreh M, Hafez E, Tousson E, Masoud A. Biochemical and molecular studies on the role of rosemary (rosmarinus officinalis) extract in reducing liver and kidney toxicity due to etoposide in male rats. Asian journal of research in Medical and Pharmaceutical Sciences. 2019;7(4):1-11.

Tawfik M, Al-Badr N. Adverse effects of monosodium glutamate on liver and kidney functions in adult rats and potential protective effect of vitamins c and e. Food and Nutrition Sciences. 2012;3(05): 651.

Farombi E, Onyema O. Monosodium glutamate-induced oxidative damage and genotoxicity inthe rat: Modulatory role of vitamin c, vitamin e and quer-cetin. Human and Experimental Toxicology. 2016;25(5): 251–259.

Ortiz G, Bitzer-Quintero O, Zrate C, Rodrguez-Reynoso S, Larios-Arceo F. Velzquez-Brizuela I, Pacheco-Moisés F, Rosales-Corral S; 2006.

Mansour |, Newairy A, Yousef M, Sheweita S. Evaluation of the effect of propolis extract on the tongue mucosa of an induced toxic rabbit by fenitrothion. Life Science Journal. 2002;10:767-775.

Al-Mamary M, Al-Habori M, Al-Aghbari A. M, Baker MM. Protective effect of n-acetyl cysteine and/or pro vitamin an against monosodium glutamate-induced cardiopathy in rats. J Pharmacol Toxicol. 2002;4:178-193.

Poli G, Cottalasso D, Pronzato M, Chiarpotto E, Biasi F, Corongiu U, Marinari G, Nanni, Dianzani M. Protective effects of alpha-tocopherol against oxidative stress related to nephrotoxicity by monosodium glutamate in rats. Toxicol Mech Methods. 1990;22:625-630.
DOI:10.3109/15376516.2012.714008

Ahmed D. Role of Eruca sativa oil treatment on profenofos supplemented female albino rats. Egypt J Exp Biol (zool.). 2014;10(2):137-143.

Tawfek N, Amin H, Abdalla A, Fargali S. Adverse effects of some food additives in adult male albino rats. Current Science International. 2015;4(4):525-537.

Mutar TF, Tousson E, Hafez E, Gazia MA, Salem SB. Ameliorative effects of vitamin B17 on the kidney against Ehrlich ascites carcinoma induced renal toxicity in mice. Environmental Toxicology. 2019;
DOI:10.1002/tox.22888

Salama A, Tousson E, Ibrahim W, Hussein W. Biochemical and histopathological studies of the ptu-induced hypothyroid rat kidney with reference to the ameliorating role of folic acid. Toxicology and Industrial Health. 2013;29(7):600-8.

Alam M, Kaur G, Jabbar Z, Javed K, Athar M. Eruca sativa seeds possess antioxidant activity and exert a protective effect on mercuric chloride induced renal toxicity. Food and Chemical Toxicology. 2007; 45(6):910-920.

Ahluwalia P, Malik V. Effects of monosodium glutamate (msg) on serum lipids, blood glucose and cholesterol in adult male mice. Toxicology Letters. 1989; 45(2-3):195-198.

Obochi G, Malu S, Obi-Abang M, Alozie Y, Iyam M. Effect of garlic extracts on monosodium glutamate (msg) induced fibroid in wistar rats. Pakistan Journal of Nutrition. 2009;8(7):970-976.

Adnan M, Hussain J, Shah M, Shinwari Z, Ullah F, Bahader A, Khan N, Khan A, Watanabe T. Proximate and nutrient composition of medicinal plants of humid and sub-humid regions in north-west pakistan. J. Med. Plant Res. 2010;4(4): 339-345.

Bukhashi E, Maliki S, Ahmed S. Estimation of nutritional value and trace elements content of carthamus oxycantha. Eruca sativa and plant ago ovanta. Pak.J.Bot. 2007;30(4):1181- 1187.

Al-Qasomi S, Al-Sohaibani M, Al-Howriny T, Al-Yahya M, Rafatullah S. Rocket (eruca sativa) a salad herb with potential gastric anti-ulcer activity. World. J. Gastroentero. 2009;15(6):1958-1965.