Evaluation of the Antidiabetic Potentials of Musa acuminata Leaves Crude Extract in Alloxan-Induced Wistar Rats

A. A. Freitas *

Department of Anatomy, Benue State University, Makurdi, Nigeria.

L. O. Onyemeh

Department of Pharmacology and Therapeutic, Delta State University, Nigeria.

P. O. Nneji

Department of Science Education, Niger Delta University, Nigeria.

O. M. Ola

Department of Physiology, Osun State University, Nigeria.

D. A. Faloye

Department of Pharmacology and Drug Development, Obafemi Awolowo University, Nigeria.

*Author to whom correspondence should be addressed.


Abstract

Context: Musa acuminata is a plant of the tropical and subtropical regions. Over the past few decades, the health benefits of Musa acuminata have received much attention. All parts of the plant, including fruits, peel, pseudo stem, corm, flowers, leaves, sap, and roots, have found their use in treating many diseases in traditional medicine.

Aim: This study was conducted to appraise the protective effect of Musa acuminata extract on alloxan-induced Diabetics Mellitus in wistar rats.

Settings and Design: This investigation was carried out using 24 Wistar rats, both males and females. The experimental models were divided into six groups; 4 rats per group.

Materials and Methods: The experimental models were divided into six groups; 4 rats per group. Alloxan was intraperitonially injected at a dose of 150 mg/kg body weight for day 1 across all groups except the positive control which is Group A together with oral administration of the aqueous extract of Musa acuminata (100 mg/kg, 200mg/kg and 300mg/kg body weight for the treatment group) and Group F was administered a standard drug. The animals were sacrificed on the 11th day under deep anesthesia with chloroform. The blood was collected by cardiac puncture and kidneys were collected for the histological profile.

Statistical Analysis: Kidney oxidative stress marker Malondialdehyde (MDA) and antioxidant enzyme activities Creatinine and Urea, were determined.

Results: The alloxan injection indicated an increase in Malondialdehyde (MDA), with a decrease in Urea and Creatinine when compared with the control. A remarkable decrease in antioxidant enzymes was also observed. Oxidant/antioxidant imbalance, alloxan-induced diabetic mellitus, and histological changes in the kidneys were reduced almost to normal by the administration of Musa acuminata.

Conclusion: Based on the current findings, Musa acuminata leaves at low dose (100 mg/kg body weight) in the alloxan Induced Wistar rat has some anti diabetics potential than at 200 mg/kg body weight, 300 mg/kg body weight and standard drug (Metformin).

Keywords: Alloxan, Musa acuminate, diabetics mellitus, kidney


How to Cite

Freitas, A. A., Onyemeh, L. O., Nneji, P. O., Ola, O. M., & Faloye, D. A. (2023). Evaluation of the Antidiabetic Potentials of Musa acuminata Leaves Crude Extract in Alloxan-Induced Wistar Rats. Asian Journal of Research in Medical and Pharmaceutical Sciences, 12(4), 7–15. https://doi.org/10.9734/ajrimps/2023/v12i4226

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References

WHO. Global center for traditional medicine in India; 2022.

Choudhury H, Pandey M, Hua CK, et al. An update on natural compounds in the remedy of diabetes mellitus: A systematic review. Journal of Traditional and Complementary Medicine. 2018;8(3):361–376. DOI: 10.1016/j.jtcme.2017.08.012

Maiese K. New insights for oxidative stress and diabetes mellitus. Oxidative Medicine and Cellular Longevity. 2015; 875961. DOI: 10.1155/2015/875961

WHO. International news room fact sheets on diabetes; 2023.

Viigimaa M, Sachinidis A, Toumpourleka M, Koutsampasopoulos K, Alliksoo S, Titma T. Macrovascular complications of type 2 diabetes mellitus. Current Vascular Pharmacology, 2020;18(2):110–116. DOI:10.2174/1570161117666190405165151

Juan J, José M. Update on the treatment of type 2 diabetes mellitus. World Journal of Diabetes. 2016;7(17):354–395. DOI: 10.4239/wjd.v7.i17.354

Glossmann HH, Lutz OM. Pharmacology of metformin - an update. European Journal of Pharmacology. 2019;865: 172782. DOI: 10.1016/j.ejphar.2019.172782

Ma R. Acarbose: An alternative to metformin for first-line treatment in type 2 diabetes. Lancet Diabetes & Endocrinology. 2014;2(1):6–7. DOI: 10.1016/S2213-8587(13)70107-4

Syed WG, Syed ASS, Vineetha M, Nazeerullah R, Riham ME, Hassaan AR. Effect of different antidiabetic medications on atherosclerotic cardiovascular disease (ASCVD) risk score among patients with type-2 diabetes mellitus: A multicenter non-interventional observational study; 2022.

DOI: 10.1371/journal.pone.0270143 PMCID: PMC9239438, PMID: 35763504

Lautie E, Russo O, Ducrot P, Boutin JA. Unraveling plant natural chemical diversity for drug discovery purposes. Frontiers in Pharmacology. 2020;11:397. DOI: 10.3389/fphar.2020.00397

Dinda B, Dinda M. Natural products in obesity and diabetes: Therapeutic potential and role in prevention and treatment. Springer. Natural products, a potential source of new drug discovery to combat obesity and diabetes: Their efficacy and multi-target actions in the treatment of these diseases. 2022:101–275.

Riaz Z, Ali MN, Qureshi Z, Mohsin M. In vitro investigation and evaluation of a novel drug based on polyherbal extract against type 2 diabetes. Journal of Diabetes Research. 2020:7357482.

DOI: 10.1155/2020/7357482

Ali S. Stereological and immunohisto-chemical study on the submandibular gland of diabetic albino rats. J. Am. Sci. 2018;14(12):24–33.

Miaffo D, Kamgue OG, Tebou NL, Temhoul CM, Kamanyi A. Antidiabetic and antioxidant potentials of Vitellaria paradoxa barks in alloxan-induced diabetic rats. Clinical Phytoscience. 2019;5:44.

Umerah NN, Asouzu AI. Evaluation of glycemic index, hypolipidemic and hypoglycemic activities of "osuune" on alloxan-induced diabetic rats; 2023. Available:https://www.sdiarticle5.com/review-history/99372

Daisy P, Kani FGJ. Hypolipidemic and hepatoprotective effects of Cassia auriculata Linn bark extracts on streptozotocin-induced diabetics in male Wister albino rats. Asian J. Pharmaceut. Clin. Res. 2013;6(2):43–48.

Johnson UA, Eze KN, John I. Modulations in anti-oxidant activities of selected gastro-intestinal tissues in alloxan- induced, silymarin treated diabetic wistar rats. Open Journal of Gastroenterology. 2019;9(5). DOI: 10.4236/ojgas.2019.95010

Sekiou O, Boumendjel M, Taibi F, Boumendjel A, Messarah M. Mitigating effects of antioxidant properties of artemisia herbal alba aqueous extract on hyperlipidemia and oxidative damage in alloxan-induced diabetic rats. Arch Physiol Biochem. 2018:1–11.

Sekiou O, Boumendjel M, Taibi F, Tichati L, Boumendjel A, Messarah M. Nephroprotective effect of artemisia herba alba aqueous extract in alloxan-induced diabetic rats. Arch Physiol Biochem. 2021:1–11.

Mahmoud E, Mahmoud M. Effect of pomegranate peel extract on submandibular salivary glands of streptozotocin-induced diabetes in rats: Histological, immunohistochemical and ultrastructural study. J. Adv. Biol. Biotechnol. 2017;13(3):1–15.

Al-Ankily M, Shamel M, Bakr M. Epidermal growth factor improves the ultra structure of sub-mandibular salivary glands of streptozotocin-induced diabetic rats - a qualitative study. Int. J. Medi. Dental Sci. 2020;9(1):1803–1810.

Elias W. The anti-diabetic effect of hibiscus cannabinus extract on the submandibular salivary gland of alloxan-induced diabetic albino rats. Int. J. Pharmaceutical Res. Allied Sci. 2020;9(2):195–202.

Salem Z, Kamel A, AbuBakr N. Salivary exosomes as a new therapy to ameliorate diabetes mellitus and combat xerostomia and submandibular salivary gland dysfunction in diabetic rats. J. Mol. Histol. 2021;52(3):467–477.

Sadeghinezhad J, Tootian Z, Javadi F. Anatomical and histological structure of the tongue and histochemical characteristics of the lingual salivary glands in the persian squirrel (Sciurus anomalus). Anat. Sci. Int. 2016;93(1):58–68.

El-Ghazawy Y, El-Zainy M, Hassan R. Histological and immunohistochemical analysis of green coffee aqueous extract effect on parotid salivary gland in streptozotocin-induced diabetic albino rats. EJH. 2020;43(3):748–762.

Das J, Parames C, Sil. Taurine ameliorates alloxan-induced diabetic renal injury, oxidative stress-related signaling pathways, and apoptosis in rats. Amino Acids. 2012;43:1509–1523.

Idonije BO, Festus O, Oluba OM. Plasma glucose, creatinine, and urea levels in type 2 diabetic patients attending a Nigerian teaching hospital. Research Journal of Medical Sciences. 2011;5(1): 1-3.

Muriach M, Flores-Bellver M, Romero F. J., Barcia JM. Diabetes and the brain: Oxidative stress, inflammation, and autophagy. Oxidative Medicine and Cellular Longevity. 2014:102158.

DOI: 10.1155/2014/102158

Thomas DD, Corkey BE, Istfan NW, Apovian CM. Hyperinsulinemia: An early indicator of metabolic dysfunction. Journal of the Endocrine Society. 2019;3(9):1727–1747.

DOI: 10.1210/js.2019-00065