Development of Phyto-cosmetic Herbal Sindoor from Rosa indica L. and Its Characterization

Akanksha Singh

Phytochemistry Division, CSIR-National Botanical Research Institute, Lucknow-226001, India.

Meenu Verma

Phytochemistry Division, CSIR-National Botanical Research Institute, Lucknow-226001, India.

Vaishali Mishra

Phytochemistry Division, CSIR-National Botanical Research Institute, Lucknow-226001, India.

K. J. Singh

Botanical Garden Division, CSIR-National Botanical Research Institute, Lucknow-226001, India.

S. K. Tewari

Botanical Garden Division, CSIR-National Botanical Research Institute, Lucknow-226001, India.

Mahesh Pal *

Phytochemistry Division, CSIR-National Botanical Research Institute, Lucknow-226001, India.

*Author to whom correspondence should be addressed.


Abstract

Aim: The objective of this research study was to develop a liquid herbal sindoor containing flower petal extract of Rosa indica L., and to evaluate the stability, antioxidant and antibacterial activities of the developed formulation.

Place and Duration of Study: The formulation was prepared in the Phytochemistry Division of CSIR- National Botanical Research Institute. The duration of the study period was 90 days.

Methodology: The crude ethanolic extract of R. indica flowers was incorporated into a base for the preparation of liquid herbal sindoor. The antioxidant activity of this herbal product was evaluated according to the DPPH radical scavenging method, and the stability study of the formulation was evaluated for 90 days. The antibacterial activity was performed on those strains of bacteria, which are responsible for several skin infections using Disc Diffusion Assay.The HPLC- DAD analysis identified the anthocyanins cyanidin 3,5-diglucoside and pelargonidin 3,5-diglucoside in R. indica, flower extract.

Results and Discussion: The formulation showed antioxidant activity and its stability was assessed in terms of pH values and color stability of the flower extract. It was observed that both these properties did not show any significant changes.

Conclusion: The prepared herbal liquid sindoor was found safe to use having a potential to substitute the toxic lead tetraoxide (Pb3O4) containing sindoor available in the market.

Keywords: Rosa indica, flower extract, formulation, antioxidant, antibacterial, pH value and color stability, lead tetraoxide


How to Cite

Singh , A., Verma , M., Mishra , V., Singh , K. J., Tewari, S. K., & Pal , M. (2023). Development of Phyto-cosmetic Herbal Sindoor from Rosa indica L. and Its Characterization. Asian Journal of Research in Medical and Pharmaceutical Sciences, 12(4), 85–93. https://doi.org/10.9734/ajrimps/2023/v12i4233


References

Shivam Samariya, Sumeet Dwivedi, Shweta Patil, Debadash panigrahi and hemant joshi: Formulation and evaluation of herbal sindoor using different natural/herbal ingredients. International Journal of Pharmacy Teaching & Practices. 2013;3:752-754

Durgesh Wadhwa, Gopal Arora. Identification of lead from sindur samples. International Journal of Innovative Research in Engineering & Management (IJIREM). 2022;9:357-360

Crepet WL, Nixon KC, Gandolfo MA. Fossil evidence and phylogeny: The age of major angiosperm clades based on mesofossil and macrofossil evidence from cretaceous deposits. American Journal of Botany. 2004; 91: 1666–1682

Thakare Priya, Ashok Rao, Deshbhratar Kiran, Suryawanshi MN. A brief review on therapeutic effects of – “ormamental plant” rose. International Journal of Ayurveda and Pharma Research. 2017;5:46-52

Yusra Safdar, Taqdees Malik. Antibacterial activity of the rose extract. Open Access Journal of Complementary and Alternative Medicine. 2020;2:194-201

Mishra RP, Mohammad Arshad, Abdul Sami. Antibacterial Properties of Rosa indica (L.) Stem, Leaves and Flowers. Journal of Pharmaceutical and Biomedical Sciences (JPBMS). 2011;12:1-3.

Prerna, Prince sharma, Sanjana Chaudhary, Jyoti Tyag. A review on antimicrobial and antioxidant activity of rosa damascene against different species of microbes. International Journal of Advance Research and Innovative Ideas in Education. 2021;7:3555-3568

Jin Hwan Lee, Hyeon-Jin Lee, Myoung-Gun Choung: Food Chemistry. 2011;129: 272-278.

Huihua Wan, Chao Yu, Yu Han, Xuelian Guo, Sagheer Ahmad, Aoying Tang, Jia Wang, Tangren Cheng, Huitang Pan, Qixiang Zhang. Flavonols and carotenoids in yellow petals of rose cultivar (Rosa ’Sun City’): A possible rich source of bioactive compounds. Journal of Agricultural and Food Chemistry. 2018;66:4171–4181

Mohammed Azhar Bintory, Seetharamu GK, Munikrishnappa PM, Ramegowda GK, Basavaraj G. Evaluation of the Colour of Dried Dutch Rose Flowers Using a Colorimeter. Journal of Horticulture. 2015;2.

Rafaela Santos de Melo, Silvio Alan Gonçalves Bomfim Reis, Amanda Leite Guimarães, Naiane Darklei dos Santos Silva, Joao Miguel Rocha, Noureddine El Aouad, Jackson Roberto Guedes da Silva Almeida. Phytocosmetic Emulsion Containing Extract of Morusnigra L. (Moraceae): Development, stability study, antioxidant and antibacterial activities. Cosmetics. 2022;9:1-12.

Blois MA. Antioxidant determination by the use of a stable free radical. Nature. 1958; 181: 1199–1200.

Amit Alexander Charan, Prerak Gupta. Comparative analysis of antibacterial, antioxidant and photosynthetic activity of Azadirachta indica, Rosa indica and Moringa oliefera cultivars. International Journal of Current Research. 2013;5:556-561.

Huihua Wan, Chao Yu, Yu Han, Xuelian Guo, Le Luo, Huitang Pan, Tangchun Zheng, Jia Wang, Tangren Cheng and qixiang zhang: Determination of flavonoids and carotenoids and their contributions to various colors of rose cultivars (Rosa spp.). Frontiers in Plant Science. 2019;10. Article 123.

Poonam Kumari, Raju DVS, Prasad KV, Kanwar Pal Singh, Supradip Saha, Ajay Arora, Firoz Hossain. Quantification and correlation of anthocyanin pigments and their antioxidant activities in rose (Rosa hybrida) varieties. Indian Journal of Agricultural Sciences. 2017;87:76-82.

Lin L, Hyeonmi H, JeehyeS, Younghwa K, Heon-Sang JL. Antioxidant activities of methanolic extracts from four different rose cultivars. Journal of Food and Nutrition Research. 2014;2:69–73.

Serup J, Jemec GBE, Grove GL. (Eds.) Handbook of Non-Invasive Methods and the Skin, 2nd ed.; Taylor & Francis. Boca Raton, FL, USA. 2006;1056.

Deepali Singhee, Adrija Sarkar. colorimetric measurement and functional analysis of selective natural colorants applicable for food and textile products. IntechOpen. 2022;1-33.

Akshada Kakade, Divya Yadav, Pranoti Bhonjale, Pallavi Velapure, Akshay Vedpathak, Prajyoti Hiware. A new approach of formulation and evaluation of herbal sindoor powder and stick from herbal Ingredients. European Journal of Biomedical and Pharmaceutical Sciences. 2016;3:251-253.

Sheema Bai, Leena Seasotiya, Anupma Malik, Pooja Bharti, Sunita Dalal: Bioactive compounds and pharmacological potential of Rosa indica L. and Psidiumguajava L. methanol extracts as antiurease and anticollagenase agents. Der Pharmacia Lettre. 2015;7:179-184.