Reduction of Salmonella enterica & Staphylococcus aureus Biofilm Development on Glass Tube by Plant Extracts
Md. Sultan Mahmud
Department of Microbiology, Jashore University of Science and Technology (JUST), Bangladesh.
Md. Ashiqur Rahman *
Department of Diagnostic, Novus Clinical Research Services Limited (NCRSL), Bangladesh.
Md. Nazmul Islam
Department of Microbiology, Jashore University of Science and Technology (JUST), Bangladesh.
Sadia Islam
Department of Pharmacology, Bangladesh University of Health Sciences (BUHS), Bangladesh.
Sajib Kumar Saha
Department of Microbiology, Jashore University of Science and Technology (JUST), Bangladesh.
Md. Taijul Islam
Department of Microbiology, Jashore University of Science and Technology (JUST), Bangladesh.
Anny Khatun
Department of Microbiology, Jashore University of Science and Technology (JUST), Bangladesh.
Silva Adhikari
Department of Microbiology, Jashore University of Science and Technology (JUST), Bangladesh.
*Author to whom correspondence should be addressed.
Abstract
The objective of the present study is to evaluate anti-biofilm effect of the water soluble plant extracts such Coccinia grandis, Terminalia arjuna, Centella asiatica against Salmonella enterica and Staphylococcus aureus biofilm. Crude water soluble extracts of respective plants with different concentration was evaluated against biofilm adopting glass tube. Then washed with crystal violet dye and PBS buffer for observing the ring formation. Biofilm inhibition study revealed water soluble plant extracts inhibited biofilm formation. In our experiment we found that the plant extracts Coccinia grandis, Terminalia arjuna & Centella asiatica gave excellent result for the reduction of biofilm of Salmonella enteric & Staphylococcus aureus. Water soluble Coccinia grandis extract is very effective for biofilm reduction than alcoholic C. grandis extracts. We measured the antiplantonic effect of these extracts by including the extracts into the nutrient agar media that containing respective organism by creating the hole into the plate then observed the result after 24-hour incubation. We also measured minimum inhibition concentration of these extracts through spectrophotometer with the help of the nutrient broth media. Bacterial motality was tested in petri plates with semi-solid medium (LB+0.4% agar) containing plant extracts and culture were inoculated in the center of the plate. This research will be very beneficial for us to reduce the pathogenic S, aureus & S. enterica biofilm by natural source especially plant extracts.
Keywords: Biofilm, Salmonella enterica, Staphylococcus aureus, plant extracts
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References
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