Bioactive Compounds and Antioxidant Potential of Aqueous and Hydroethanolic Extracts of Cola lateritia Fruit Parts

Ferdinand Lanvin Edoun Ebouel *

Institute of Medical Research and Medicinal Plants Studies (IMPM), P.O Box: 13 033, Yaoundé, Cameroon and Laboratory of Metabolic Studies, Centre of Food, Food Safety and Nutrition Research (CRASAN), P.O Box: 13 033, Yaoundé, Cameroon.

Kevin Fabrice Paul Mandeng

Institute of Medical Research and Medicinal Plants Studies (IMPM), P.O Box: 13 033, Yaoundé, Cameroon and Food Technology Development Laboratory, Centre of Food, Food Safety and Nutrition Research (CRASAN), P.O Box: 13 033, Yaoundé, Cameroon.

Evrard Medjo Kouopestchop

Institute of Medical Research and Medicinal Plants Studies (IMPM), P.O Box: 13 033, Yaoundé, Cameroon and Food Technology Development Laboratory, Centre of Food, Food Safety and Nutrition Research (CRASAN), P.O Box: 13 033, Yaoundé, Cameroon.

Joelle Ornella Tseno Tchuenkam

Laboratory of Studies and Food Control, Centre of Food, Food Safety and Nutrition Research (CRASAN), P.O Box: 13 033, Yaoundé, Cameroon.

Foura Woumdi

Laboratory of Nutrition and Nutritional Biochemistry (LNNB), Faculty of Sciences, University of Yaoundé 1, P.O Box: 812, Yaoundé, Cameroon.

Françoise Ntentie

Laboratory of Nutrition and Nutritional Biochemistry (LNNB), Faculty of Sciences, University of Yaoundé 1, P.O Box: 812, Yaoundé, Cameroon.

Ann-Mary Mbong

Laboratory of Nutrition and Nutritional Biochemistry (LNNB), Faculty of Sciences, University of Yaoundé 1, P.O Box: 812, Yaoundé, Cameroon.

Mélanie Ngondam

Institute of Medical Research and Medicinal Plants Studies (IMPM), P.O Box: 13 033, Yaoundé, Cameroon and Laboratory of Metabolic Studies, Centre of Food, Food Safety and Nutrition Research (CRASAN), P.O Box: 13 033, Yaoundé, Cameroon.

Jean Marie Gabriel Medoua

Institute of Medical Research and Medicinal Plants Studies (IMPM), P.O Box: 13 033, Yaoundé, Cameroon.

*Author to whom correspondence should be addressed.


Abstract

Generalities: Cola lateritia (C. lateritia) is an under-valorized (neglected and unutilized) plant traditionally used in certain Cameroonian villages as medicine to manage certain diseases. The present work aimed to assess the phytochemical composition and the antioxidant potential of different parts of C. lateritia fruits.

Methods: The fresh fruits of C. lateritia were purchased in October 2022 at Mfoundi-Market (Yaoundé 5, Centre Region of Cameroon), washed, sliced, dried, and powdered before being extracted with distilled water for the aqueous extract (AE) and ethanol/distilled water (70:30, v/v) for the hydro ethanolic extract (HEE). The bioactive compounds (polyphenols, saponins, tannins, flavonoids, and alkaloids) of the different extracts of fruit were quantified using standard methods. The Antioxidant potential of the different extracts was assessed using various methods [scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals, Total Antioxidant Capacity (TAC), and Ferric Reducing Antioxidant Power (FRAP)].

Results: The results indicated that the different extracts of C. lateritia fruit contain phytochemicals [polyphenols, flavonoids, tannins, saponins and alkaloids]. The AE of the skin (AE-sCLf) presented the highest contents (590.33 µg CaE/g DM, 191.73 µg QE/g DM and 945.33 µg  SaE/g DM) for polyphenols, flavonoids and saponins respectively while, the AE of pulp  (AE-PCLf) showed the highest content in alkaloids (121.59 µg QiE/g DM). The lowest tannin content was also reported in the AE of pulp (2.02 µg CaE/g DM). The extracts of C. lateritia exhibited a good radical scavenging activity of DPPH (with an inhibition percentage of 74.084 and 69.40% respectively for HEE-SCLf and AE-sCLf at 4 mg/mL). It’s reducing power towards molybdate and iron was concentration-dependent with values ranging from 0.116 to 2.393 μg AAE/g DM for TAC and from 0.0015 to 0.0032 μg AAE/g DM for FRAP, respectively. The highest iron-reducing activity of 941.410 µg AAE/g DM was observed with AE-sCLf at 4 mg/mL. The principal component analysis (PCA) showed a strong positive correlation between Bioactive and antioxidant activities.

Conclusion: The C. lateritia fruit extracts (AE and HEE) contain various bioactive compounds, which exhibit good antioxidant activities through different mechanisms. It suggests C. lateritia as a potential nutritious food as well as functional food useful for the prevention of management of cardiometabolic diseases.

Keywords: Cola lateritia, antioxidant potential, bioactive compounds, aqueous extract, hydro-ethanolic extract


How to Cite

Ebouel , Ferdinand Lanvin Edoun, Kevin Fabrice Paul Mandeng, Evrard Medjo Kouopestchop, Joelle Ornella Tseno Tchuenkam, Foura Woumdi, Françoise Ntentie, Ann-Mary Mbong, Mélanie Ngondam, and Jean Marie Gabriel Medoua. 2023. “Bioactive Compounds and Antioxidant Potential of Aqueous and Hydroethanolic Extracts of Cola Lateritia Fruit Parts”. Asian Journal of Research in Medical and Pharmaceutical Sciences 12 (4):145-55. https://doi.org/10.9734/ajrimps/2023/v12i4238.

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References

Kouogueu SG, Nguedjo WM, Dibacto RE, Nseme Mboma YD, Djouka Nembot PM and Takuissu Nguemto GRx. Effect of two drying methods on the bioactive cashew apple varieties consumed in the city of garoua (Northern Cameroon). European Journal of Medicinal Plants. 2021;32(12): 64-77.

Aluko RE. Functional Foods and Nutraceuticals; Springer: Berlin/ Heidelberg, Germany; 2012.

Maury GL, Rodríguez DM, Hendrix S, Escalona Arranz JC, Fung Boix Y, Ochoa Pacheco A, Díaz JG, Morris-Quevedo HJ, Dubois AF, Aleman EI, Beenaerts N, Méndez-Santos IE, Orberá Ratón T, Paul Cos P and Ann Cuypers A. Antioxidants in Plants: A Valorization Potential Emphasizing the Need for the Conservation of Plant Biodiversity in Cuba. Antioxidants (Basel). 2020;9(11):1048.

Liu RH. Health-promoting components of fruits and vegetables in the diet. Advanced Nutrition. 2013;4(3):384S–392S.

Donno D, Turrini F. Plant foods and underutilized fruits as source of functional food ingredients: Chemical Composition, Quality Traits, and Biological Properties. Foods. 2020;9(10):1474.

Ekalu A, Habila JD. Phytochemistry, pharmacology and medecinal uses of Cola (Malvaceae) family: A review. Medecinal Chemistry Research. 2020; 29:2089-2105.

Singleton V, Rossi J. Colorimetry of total phenolics with phosphomolydic-phosphotungstic acid reagents. Am J Enology Viticult. 1965;16:144-158.

Aiyegoro OA, Okoh AI. Preliminary phytochemical screening and in vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC. BMC Complement Altern Med. 2010;10:21.

Bainbridge Z, Tomlins K, Willings K, Westby A. Methods for assessing quality characteristics of non-grain starch staple. Part 4 advanced methods. National ressources institute. University of Greenwich. 1996;1:43–79.

Singh DK, Srivastva B, Sahu A. Spectrophotometric determination of Rauwolfia alkaloids, estimation of reserpine in pharmaceuticals. Analytical Sci. 2004;20:571-573.

Sanchez-Moreno C, Larrauri J, Saura-Calixto F. A procedure to measure the antiradical efficiency. Sci Food Agric. 1998; 76:270-276.

Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem. 1999;269:337-341.

Oyaizu M. Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Jap J Nutri. 1986;44: 307-315.

Edoun EFL, Tchuente TBR, Dibacto KER, Mouafo TH, Ndzana AAC, Tchuentchieu KAD, Medoua NG. Phytochemical screening and antioxidant potential of aqueous extracts of Milletia laurenti, Lophira alata and Milicia excels, commonly used in the Cameroonian pharmacopoeia. European Journal of Medicinale Plants. 2020:58837.

ISSN 2231-0894, NLM ID 101583475.

Mbong AM-A, Edoun EFL, Manga Ngandi LC, Fotso YJA, Orang Orang R, Fotso Tienoue HM, Ngalla Nwang F, Ngondi JL and Oben J. Comparative Study of the protective effect of Cola anomala and Coffea arabica Against Induced Toxicity in Rats. Journal of Food Research. 2020; 9(5).

ISSN 1927-0887; E-ISSN 1927-0895.

Konczak I, Zabaras D, Dunstan M, Aguas P. Antioxidant capacity and phenolic compounds in commercially grown native Australian herbs and spices. Food Chem. 2010;122:260–266.

Mariya Saani, Reena Lawrence. Evaluation of pigments as antioxidant and antibacterial agents from beta vulgaris Linn. International Journal of Current Pharmaceutical Research. 2016;8(3).

Parente CP, Reis Lima MJ, Teixeira-Lemos E, Moreira MM, Barros AA, and Guido LF. Phenolic Content and Antioxidant activity determination in broccoli and lamb’s lettuce. International Journal of Biological, Biomolecular, Agricultural, Food and Biotechnological Engineering. 2013;7(7).

Altemimi A, Lakhssassi N, Baharlouei A, Watson DG, Lightfoot DA. Phytochemicals: Extraction, Isolation, and Identification of Bioactive Compounds from Plant Extracts. Plants. 2017;6:42.

Sun JS, Tsuang YW, Chen JJ, Hang YS, Lu FJ. An ultra-weak chemiluminescence study on oxidative stress in rabbits following acute thermal injury. Burns. 1998;24:225-231.

Van Ngo T, Scarlett CJ, Bowyer MC, Ngo PD, Van Vuong Q. Impact of Different Extraction Solvents on Bioactive Compounds and Antioxidant Capacity from the Root of Salacia chinensisL. J Food Qual. 2017:1-8.

Zinmmerman M, Snow B. An introduction to Nutrition. Creative commons. 2012: 22.

Barchan A, Bakkali M, Arakrak A, Pagán R, Laglaoui A. The effects of solvents polarity on the phenolic contents and antioxidant activity of three Mentha species extracts. Int. J. Curr. Microbiol. Appl. Sci. 2014;3:399–412.

Iloki-Assanga SB, Lewis-Luján LM, Lara-Espinoza CL, Gil-Salido AA, Fernandez-Angulo D, Rubio-Pino JL, Haines DD. Solvent effects on phytochemical constituent profiles and antioxidant activities, using four different extraction formulations for analysis of Bucida buceras L. and Phoradendron californicum. BMC Res. Notes 2015;8:396.

Brahmi F, Mechri B, Dabbou S, Dhibi M, Hammami M. The efficacy of phenolics compounds with different polarities as antioxidants from olive leaves depending on seasonal variations. Ind. Crops Prod. 2012;38:146–152.

Ultee A, Bennik M, Moezelaar R. The phenolic hydroxyl group of carvacrol is essential for action against the food-borne pathogen Bacillus cereus. Appl. Environ. Microbiol. 2002;68:1561–1568.