In Rodents, Methanol Extract from Ritchiea capparoides leaf has Analgesic, Anti-Inflammatory, and Antipyretic Properties
Okorie O
Department of Pharmacology and Therapeutics, Faculty of Basic Clinical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nigeria.
Akuodor GC
Department of Pharmacology and Therapeutics, Faculty of Basic Clinical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nigeria.
Ifediba CE
Department of Pharmacology and Therapeutics, Faculty of Basic Clinical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nigeria.
Ofor CC
Department of Pharmacology and Therapeutics, Faculty of Basic Clinical Sciences, Ebonyi State University, Abakaliki, Nigeria.
Afonne OJ *
Department of Pharmacology and Therapeutics, Faculty of Basic Clinical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Background: Ritchiea capparoides leaves are traditionally used in Southern Nigeria to cure fever, snake bites, aches, and malaria. Consequently, the analgesic, anti-inflammatory, and antipyretic properties of Ritchiea capparoides leaf ethanol extract were assessed in rodents in this work.
Methods: Using acetic acid and tail immersion models in mice, the analgesic effect was investigated, and xylene, egg-albumen, brewer's yeast, and dinitrophenol models were used to investigate the anti-inflammatory and antipyretic properties in mice and rats at doses of 125 mg/kg, 250 mg/kg, and 500 mg/kg of the methanol extract. The plant's leaf extract was also subjected to oral acute toxicity testing and phytochemical screening.
Results: At p<0.05 and p<0.01, the methanol leaf extract and the common medication (aspirin) considerably reduced the amount of writhes brought on by acetic acid. The reaction times of the tested agent's standard and extract groups significantly increased. The extract significantly reduced oedema in the egg-albumin-induced paw oedema model, with dose-related inhibition of p<0.05 and p<0.01, similar to aspirin. When compared to dexamethasone, Ritchiea capparoides leaf extract likewise showed a significant p<0.05 and p<0.01 effect in the xylene-induced mouse ear oedema test. Rats with pyrexia caused by dinitrophenol and Brewer's yeast both showed a statistically significant decrease in rectal temperatures. Alkaloids, saponins, tannins, flavonoids, terpenoids, steroids, and cardiac glycosides are all present in the methanol leaf extract. It was discovered that the oral acute toxicity testing were more than 5000 mg/kg.
Conclusion: The findings supported the traditional use of R. capparoides as a medicine by demonstrating the plant's possible analgesic, anti-inflammatory, and antipyretic properties at the tested concentrations of methanol leaf extract.
Keywords: Ritchiea capparoides, leaf extract, antipyretic, analgesic, anti-inflammatory
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Javed F, Jabeen Q, Aslam N, and Awan AM. Pharmacological evaluation of analgesic, anti-inflammatory and antipyretic activities of ethanolic extract of Indigofera argentea burm. F. J. Journal of Ethnopharmacology. 2020;259:112966.
Akuodor GC, Anyalewechi NA, Udoh FV, Ikoro NC, Akpan JL, Gwotmut MD, Pharmacological evaluation of Verbena hastate leaf extract in the relief of fever. Advanced Pharmacology and Toxicology. 2011;12(3):1-8.
Dutta T, Paul A, Majumder M, Sultan RA, Emran TBJB. Pharmacological evidence for the use of Cissus assamica as a medicinal plant in the management of pain and pyrexia. Biochemical Biophysics Report. 2020;21:100715.
Ravelo Y, Molina V, Carbajal D, Fernández L, Fernández JC, Arruzazabala ML, Más R. Evaluation of anti-inflammatory and anti-nociceptive effects of D-002 (beeswax alcohols) Journal of Natural Medicine. 2011;65(2):330-335.
Anowi CF, Utoh-Nedosa UA, Onyegbule AF, Oche G. Antimicrobial activity of methanolic extract of the leaves of Ritchiea longipedicellata. Fam Capparidaceae International Journal of Pharmacy. 2012; 2(2):287–293
Sofidiya MO, Osuala EC, Onyemaechi CJ, Fageyinbo MS. Evaluation of antinociceptive activity of Ritchiea longipedicellata (Capparaceae) leaf extract in mice, Journal of Herbs, Spices Medicinal Plants. 2018;24:(4):356-367
Ajaiyeoba EO, Okogun JI. Antheimintic activity of roots of Ritchiea capparoides var. longipedicellata. Phytotherapy Research. 1996;10:436–437
Ogbunugafor HA, Okochi VI, Okpuzor J, Emeka P. Tolerance and antiplasmodial screening of Ritchea longipedicellata in Plasmodium berghei. Journal of Biochemistry. 2008;20(1):23–27
Taiwo BJ, Akinkunmi EO, Omisore NO. Antimicrobial and Antiplasmodial activities of a Quaternary compound from Ritchiea capparoides var Longipedicullata. African Journal of Traditional and Complementary Alternative Medicine 2013;10(b):528-531.
Ajaiyeoba EO, Rahman AU, Choudhary IM. Preliminary antifungal and cytotoxicity
studies of extracts of Ritchiea capparoides var. longipedicellata. Journal of Ethnopharmacology. 1998;62:243–246.
Neuwinger HD. African traditional medicine: A dictionary of plant use and applications. Medpharm Scientific, Stuttgart, Germany 2000;589.
Evans WC. Trease and Evans Pharmacognosy. 15th Edn., Reed Elsevier India Pvt. Ltd., New Delhi, India 2005;174: 224-535.
Lorke D. A new approach to practical acute toxicity testing. Archiveof Toxicology. 1983;54:275–287.
Essien AD, Edidara Thomas, Essiet GA, Akuodor GC. Anti-inflammatory, antipyretic and anti-nociceptive activities of the ethanol stem bark extract of Salacia lehmbachii. British Journal of Pharmacology and Toxicology. 2017;8(2): 9-16.
Akuodor GC, Essien AD, Udia PM, David-Oku E, Chilaka KC, Asika EC, Nwadum SK. Analgesic, Anti-inflammatory and Antipyretic potential of the stem Bark Extract of Stachytarpheta indica. British Journal of Pharmacology and Toxicology. 2015;6(1):16-21.
Essien AD, Essiet GA, Akuodor GC, Akpan JL, Chilaka KC, Bassey AL. Pharmacological evaluation of the aqueous stem bark extract of Bombax buonopozense in the relief of pain and fever. African Journal of Pharmacy and Pharmacology. 2016;10(5):59-65.
Akuodor GC, Ohadoma SC, Ofor CC, Megwas AU, Chukwu LC, Ramalan MA, Okorafor DO, Chilaka KC. Anti-nociceptive, anti-inflammatory and antipyretic activities of the ethanol root bark extract of Salacia lehmbachii in rats and mice. International Journal of Basic and Clinical Pharmacology. 2021;10(6) :614-620
Ohanme EO, Etu KE, Akuodor GC, Nwakelu B. Ofor CC. Evaluation of Anti-Inflammatory, Anti-Nociceptive and Antipyretic Properties of the Ethanol Leaf Extract of Celosia Leptostachya in Rats and Mice. International Journal of Pharmacy and Pharmaceutical Research. 2022;25 (4):284-298.
Okokon JE, Nwafor PA. Anti-inflammatory, analgesic and antipyretic activities of ethanolic root extract of Croton zambesicus. Pakistan Journal of Pharmaceutical Sciences. 2010;23(4):385-92.
Shaa KK, Oguche S, Watila IM, Ikpa TF. In vitro antimalarial activity of the extracts of
Vernonia amygdalina commonly used in traditional medicine in Nigeria. Journal of Science World 2011;6(2):5-9.
Mbiantcha M, Kamanyi A, Teponno RB, Tapondjou AL, Watcho P, Nguelefack T. Analgesic and Anti-Inflammatory Properties of Extracts from the Bulbils of Dioscorea bulbifera L. var Sativa (Dioscoreaceae) in Mice and Rats. Evidence Based Complementary and Alternative Medicine. 2011:912935.
Nkeh CNB, Bekwa PCM, Ndebia JE, Kayo M, Mbafor TJ, Iputo EJ. Analgesic and anti- inflammatory properties of Oxyanthus unilocularis. Journal of Medicinal Plants. 2010;4(10):932-9.
Yam MF, Loh YC, Oo CW, Basir R. Overview of neurological mechanism of pain profile used for animal "pain-like" behavioral study with proposed analgesic pathways. International Journal of Molecular Sciences. 2020;21(12):4355.
Ezeja M, Ezeigbo I, Madubuike KG. Analgesic activity of the methanolic seed extract of Buchholzia coriacea. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2011;2(1):187- 93.
Kumar A, Agarwal K, Kumar MA, Shanker K, Bushra U, Tandon S, Bawankule DU. Pharmacological and phytochemical evaluation of Ocimum sanctum root extract for its anti-inflammatory, analgesic and antipyretic activities. Pharmacognosy Magazine. 2015;11(42):217-24.
Abotsi WKM, Lamptey SB, Afrane S, Boakye-Gyasi E, Umoh RU, Woode E. An evaluation of the anti-inflammatory, antipyretic and analgesic effects of hydroethanol leaf extract of Albizia zygia in animal models. Pharmaceutical Biology. 2017;55(1):338-348.
Xu Z, Zhou J, Cai J, Zhu Z, Sun X, Jiang C. Antiinflammation effects of hydrogen saline in LPS activated macrophages and carrageenan induced paw oedema. Journal of Inflammation. 2012;9:2.
Abdulkhaleq LA, Assi MA, Abdullah R, Zamri-Saad M, Taufiq-Yap YH, Hezmee MNM. The crucial roles of inflammatory mediators in inflammation: A review. Journal of Veterinary world. 2018; 11(5):627-35.
David OE, Akuodor GC, Edet EE, Ogbuji GK, Obiajunwa OJI, Aja DOJ. Antinociceptive, antiinflammatory and antipyretic effects of ethanolic root bark extract of Icacina senegalensis in rodents. Journal of Applied Pharmaceutical Science. 2016;(02):104-3.
Megwas1 AU, Akuodor GC, Chukwu LC, Aja DO, Okorie EM, Ogbuagu EC. Analgesic, antiinflammatory and antipyretic activities of ethanol extract of Annona senegalensi leaves in experimental animal models. International Journal of Basic and Clinical Pharmacology. 2020; 9(10):1477-84.
Ajayi AM, Tanayen JK, Magomere A, Ezeonwumelu JOC. Antinociceptive and anti-inflammatory effects of aqueous extract of Chenopodium opulifolium schrad leaves. Journal of Intercultural Ethnopharmacology. 2016;6(1):14-21.
Tarkang PA, Okalebo FA, Siminyu JD, Ngugi WN, Mwaura AM, Mugweru J, Agbor JA, Guantai AN. Pharmacological evidence for the folk use of Nefang: antipyretic, anti-inflammatory and antinociceptive activities of its constituent plants. BMC complementary and alternative Medicine. 2015; 15:174.
Sherif AE, Sajid-ur-Rehman M, Asif M, Qadeer I, Khan KR. Anti-inflammatory, analgesic, and antipyretic potential of Oxystelma esculentum (L. f.) Sm. using in vitro, in vivo, and in silico studies.Frontier Pharmacology. 2024; 14:1326968.
Agoreyo BO, Okoro NC, Choudhary MI. Preliminary phytochemical analysis of two varieties of Adenia lobate and the antioxidant activity their various solvent fractions. Bayero Journal of Pure and Applied Science. 2012; 5(1):182-6.
Savithramma M, Rao ML, Suhrulatha D. Screening of medicinal Plants for secondarymetabolites. Middle East Journal Scientific Research. 2011; 8(3): 579-84.