Investigating the Protective Effects of Ascorbic Acid Against Paracetamol-induced Liver Toxicity in Wistar Rats
Udi, O. A. *
Department of Human Anatomy, Federal University Otuoke, Bayelsa State, Nigeria.
Moronkeji, A.
Department of Medical Laboratory Science, University of Medical Science, Ondo State, Nigeria.
Nwaokoro, I. C.
Department of Human Anatomy, University of Delta, Agbor, Delta State, Nigeria.
Ekakite, O. O.
Department of Human Physiology, Federal University Otuoke, Bayelsa State, Nigeria.
Okoro, G. O.
Department of Human Anatomy, Achievers University, Owo, Ondo State, Nigeria.
Oshojobi, O. M.
Department of Medical Laboratory Science, Achievers University, Owo, Ondo State, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Paracetamol is one of the most widely used analgesics and antipyretics but can cause severe liver damage at high doses. This study explores the potential protective effects of ascorbic acid (vitamin C) against paracetamol-induced hepatotoxicity in Wistar rats. For this experimental study, 25 mature male Wistar rats, weighing 140–180g, were divided into five groups: Group 1 stands for the Normal Control. Group 2 received 25 mg of PCM for 21 days, whereas Group 3 received 500 mg of ascorbic acid. Furthermore, after PCM was given, rats in groups 4 and 5 were given 500 mg and 1000 mg of ascorbic acid, respectively. Following PCM and Ascorbic acid treatment, biochemical parameters and histoarchitectural studies were examined. Histopathological examination of liver tissue was performed to evaluate cellular architecture and damage. Results indicated that treatment with ascorbic acid significantly mitigated the elevation of liver enzymes and histopathological changes compared to the paracetamol-only group. In conclusion, the research underscores the potential role of ascorbic acid in safeguarding liver function.
Keywords: Paracetamol, ascorbic acid, hepatotoxicity, liver injury, Wistar rats