Evaluation of In vitro Antioxidant and Qualitative Phytochemical Analysis of Methanol Leaf Extract of Fadogia cienkowskii Scheinf Fam. Rubiaceae
Asian Journal of Research in Medical and Pharmaceutical Sciences,
Aim: Fadogia cienkowskii Scheinf has been used in ethno medicine for the treatment of pyrexia, convulsion in children, inflammation and malaria. The aim of this work is to ascertain the presence of some phytochemicals in the leaf of Fadogia cienkowskii Scheinf and to investigate the antioxidant property of the methanol leaf extract of the plant.
Study Design: This work was designed to investigate the presence of some phytochemicals in the plant and to further evaluate the antioxidant property of the methanol leaf extract of the plant using three models.
Methodology: The pulverized leaves (1 kg) was extracted with 5.0 L of 95% methanol employing cold maceration technique. The extract obtained was dried using rotatory evaporator.
Qualitative and quantitative phytochemical evaluations were carried out using standard methods. Antioxidant capacity was investigated using 2,2 diphenyl-1- picrylhydrazyl, ferric reducing antioxidant power and total antioxidant capacity models.
Results: The percentage yield of extract is 20%. Flavonoids, saponins, tannins, glycosides, terpenoids, alkaloids and steroids were present in the extract. Total phenol concentration was 0.55+ 0.13 ug/ml. The amount of flavonoid was 3.28+0.01 g by gravimetry with sample weight of 5+0.02 gm. The antioxidant activity of the plant methanol leaf extract showed maximal effect as demonstrated by its EC 50 of 3.811+0.03 ug/ml compared with the ascorbic acid standard 4.989+0.02 ug/ml using DPPH method. This result was validated by the FRAP and TAC models.
Conclusion: The presence of polyphenolic biomolecular compounds in the extract confirms the use of the plant in Ethnomedicine.
How to Cite
Huang YZ, Edwards MJ, Rounis E, Bata KP, Rothwell JC. Theta burst stimulation of the human motor cortex, Neuron 2005; 45(2): 201-206.
Zelko IN, Mariana TJ, Folz RJ. Superoxide dismutase multigene family: a comparison of the Cu-Zn-SOD (SOD1), Mn-SOD (SOD2), and EC-SOD (SOD3) gene structures, evolution and expression. Free Radical Biol. Med. 2002;33(3):337-349.
Trease and Evans Pharmacognosy. Raj K Amal Electric press. Delhi: India Edition. 15;516-536.
Shumaila Jan, Muhammad Rashid Khan, Ambreen Rashid, Jasia Bokhara. Assessment of antioxidant potential, total phenols and Flavonoids of different solvent fractions of Monotheca buxifolia fruit. Osang PUB. Health Res. Perspect. 2013; 4(5):246-254.
Yakubu MA, A Kanji MA, Oladiji AT. Aphrodisiac potential of the aqueous extract of Fadogia agrestis in male albino rat. Asian Androl. 2005;7(4):399-404.
Evans WC. Trease and Evans pharmacognosy. W.B Sunders ltd. London. 2002;32-33, 95-99.
Shumaila Jan, Muhammad Rashid Khan, Ambreen Rashid, Jasia Bokhari. Assessment of antioxidant potential, total phenolics and flavonoids of different solvent fractions of Monotheca buxifolia Fruit. Osong Pub. Med. Res. Perspect. 2013;4(5):246-254.
Agbafor KN, Nwachukwu N. Phytochemical analysis and antioxidant property of Vitex doniana and Mucuna pruriens. Biochem. Res. Int. 2011;art.ID 459839, 4.
Yen GC, Duh D, Tsai. Relationship between antioxidant activity and maturity of peanut Hulls, J. Agric. Chem. 1993;41(1): 67-70.
Craig WJ. Health promoting properties of herbs. Amer. Clin. Nutr. 1999;70(3):491-499.
Varadarajan P, Rathinsway G, Ansarulla D. Antimicrobial properties of and phytoconstituents of Rheon disider. Ethnobot. Leafl. 2008;12:841-845.
Sowetan. Pharmacological and other beneficial effects of antinutritional factors in plants-a review. Afric. J. of Biotech. 2008; 7(25):4713-4726.
Cao G, Sofie E, Prior RL. Antioxidant and pro-oxidant behavior of flavonoids: Structure activity relationships. Free Radic. Biol & Med. 1997;22(5):749-760.
Gordon MF. The mechanism of antioxidant action in vitro In: BJF Hudson Ed, food antioxidants. Elsevier Applied Science. London. 1993;1-8.
Litvinenko G, Ingold KU. Abnormal solvent effects on Hydrogen abstraction 1. The reactions of phenols with 2, 2-diphenyl -1-picryl hydra Zyl (dpph in alcohol). J. Org. Chem. 2003;3433-3438.
Blois M. Antioxidant determination by the use of a stable free radical. Nature. 1958; 181:1199-1200.
Edenta C, James DB, Owolabi OA, Okoduwa SIR. Hypoglycemic effects of aqueous extract of three cultivars of Musa sapientum fruit peel on poloxamer-407 induced induced hyperlipidemic wistar rats.
Ramakrishnan Baskar, Selvaraj Shrisakthi, Babu Sathya Priya, Radhakrishnan Nithya, Palanisamy Poongodi. Antioxidant potential of peel extracts of banana varieties (Musa sapientum), food and nutria. Sci. 2011;2:1128-1133.
Hangerman AE, Riedl KM, Jones GA, Jovick KN, Richard NT, Hartsfield PW, Reichel TW. High molecular weight plant phenolics (tannins) as biological antioxidants. Agric. Food. Chem. 1998; 46(11998):1887-1892.
Warner DDM, Burton GW, Ingold KU, Locke SJ. Feb Letter. 1985;187:33.
Lee K, Zivin J, Ashwood T, Davalos A, Davis S, Diener H, Grotta. NXY-059 for acute ischemic stroke. Engel Med. 2006; 354(6):588-600.
Yamaguchi T, Takakura H, Matoba T, Terao T. HPLC evaluation of free radicals from plants. Bios Biotech. Biochem. 1998; 62(6):1201-1204.
Lawson LD, Ransom DK, Hughes BG. Characterization of formation of Allicin and other thiosulfinates from garlic. Planta Med.1992;58:345-350.
Prior RL, Wux Shaic K. Standardization methods for determination of antioxidant capacity and phenolics in foods and dietary supplements. Agric. Food. Chem. 2005; 53(10):4290-4302.
Lennon SV, Martin SJ, Colter TG. Dose dependent induction of apoptosis in human proliferation. Diverging stimuli, cell widely lines tumor 14. 1991;24:1365-2184.
Lilli JL, Becks LL, Dombrowski MI, Hinshaw DB. ATP converts necrosis to apoptosis in cells. Radical endothelial injury; 1998.
Abstract View: 55 times
PDF Download: 24 times