Hepatic Enzyme Effects of an Imperata cylindrica Extract
Asian Journal of Research in Medical and Pharmaceutical Sciences,
Page 32-40
DOI:
10.9734/ajrimps/2021/v10i330168
Abstract
This study was performed to investigate the effects of aqueous Imperata cylindrica root extract on hepatic enzyme levels of alloxan-induced diabetic male Wistar rats. Forty (48) male wistar rats were divided into six groups consisting of eight animals each. Diabetes mellitus was induced using intraperitoneal administration 150 mg/kg body weight of alloxan and treatment was carried out for a period of 28 days. The first group served as the normal control and received only feed and water ad libitum. In Group 2 were diabetic rats without treatment with extracts. Group 3: diabetic rats treated with 200 mg/kg aqueous Imperata cylindrica root extract. Group 4: diabetic rats treated with 400mg/kg aqueous Imperata cylindrica root extract. Group 5: diabetic rats treated with 600mg/kg ethanol extract of aqueous Imperata cylindrica root extract. While Group 6 was diabetic rats treated with 0.5mg/kg Glibenclamide. The liver enzymes alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase levels were significantly (p < 0.05) changed in rats treated with Alloxan (150mg/kg b.w.) while treatment with the respective dosages of extracts significantly changed the levels of these parameters to normal. The results obtained indicate that the different doses of aqueous Imperata cylindrica root extracts were beneficial in mending damages to the liver caused by Alloxan monohydrate in the male wistar rats.
Keywords:
- Aqueous Imperata cylindrica root extract
- diabetic rats
- Hepatoprotective
- Alanine aminotransferase
- Aspartate aminotransferase
- alkaline phosphatise
- acid phosphatase
How to Cite
Nwokike, M. O., Ghasi, S. I., Ogbuagu, E. C., Ezenwaeze, M. N., & Ajirioghene, A. E. (2021). Hepatic Enzyme Effects of an Imperata cylindrica Extract. Asian Journal of Research in Medical and Pharmaceutical Sciences, 10(3), 32-40. https://doi.org/10.9734/ajrimps/2021/v10i330168
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Szkudelski T. The mechanism of alloxan and streptozotoxin action in B cells of the rat pancreas. Physiol. Res. 2001: 537-546.
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Mbaka GO, Owolabi MA. Evaluation of haematinic activity and subchronic toxicity of Sphenocentrum jollyanum (menispermaceae) seed oil. European Journal of Medicinal Plants 2011;1(4):140-152.
Aitken RJ, Roman SD. Antioxidant systems and oxidative stress in the testes. Oxid Med Cell Longev. 2008;1(1):15-24.
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Nyblom H, Björnsson E, Simrén M, Aldenborg F, Almer S, Olsson R. The AST/ALT ratio as an indicator of cirrhosis in patients with PBC. Liver Int. 2006;26 (7):840-5.
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McClatchey KD. Clinical laboratory medicine. 1st ed. Philadelphia: Lippincott Williams & Wilkins; 2002.
Gamal A. Mohamed et al. Chemical Composition and Hepato-protective activity of Imperata cylindrica. Beauv Research Article. 2009;5(17):28-36.
Saei H, Azarmi M, Dehghan G, Salmasi SZ, Zeinali S. Hepatoprotective effect of ethanolic extract of Lavandula officinalis L. in alloxan-induced diabetic rats. Thrita. 2016;5(2):e35306.
Ayurvedic Pharmacopoeia of India; 2006.
Nwokike MO, Ghasi SI, Anusiem CA, Ogbonna AO. Effect of Imperata cylindrica roots extracts on some cardiovascular parameters of diabetic wistar rats. J Pharma Pharma Sci. 2020;4:190. DOI: 10.29011/2574-7711.100090.
Nwokike MO, Ghasi SI, Ogbonna AO, Anusiem CA. The effect of Imperata cylindrica root aqueous extracts on serum testosterone levels of hyperglycemic rats. J Pharma Pharma Sci. 2020;4:187. DOI: 10.29011/2574-7711.100087
Lalchhandama, Kholhring, Lalthanpuii PB. Imperata cylindrica: a noxious weed of pharmacological potentials. In book: Advances in Engineering Research Perspective and Trends in the Development of Science Education and Research (pp.173-177) Publisher: Atlantis Press, Paris, France. DOI 10.2991/msc-18.2018.28.
Okey-Nzekwe C, Ekwonu Agatha, Egwuatu C. Pharmacological activities of compounds of leaves and roots of Imperata cylindrica with its antimicrobial and structural elucidation. American Academic & Scholarly Research Journal 2019, 11. 20-35.
Zoarilala Rinah Razafindrakoto, Nantenaina Tombozara, Dario Donno, Giovanni Gamba, Ninà Robertina Nalimanana, Dina Andriamahavola Rakotondramanana, Charles Andrianjara, Gabriele Loris Beccaro, David Ramanitrahasimbola. Antioxidant, analgesic, anti-inflammatory and antipyretic properties, and toxicity studies of the aerial parts of Imperata cylindrica (L.) Beauv., South African Journal of Botany. 2021;142:222-229. ISSN 0254 6299. DOI:https://doi.org/10.1016/j.sajb.2021.07.004.
Mak-Mensah EE, Terlabi EO, Komlaga G. Antihypertensive action of ethanolic extract of Imperata cylindrica leaves in animal models. Journal of Medicinal Plants Research. July 2010;4(14): 1486-1491 , / DOI: 10.5897/JMPR09.298
Tessa V. Valle, Kaiser German L. Gonzalez, Joan M. Villegas, Honeyliza L. Baloloy, Noel Raven E. Magtaos: Imperata cylindrica (Cogon Grass) As Anticoagulant Agent: Haematological Effect On Human Blood / Thesis; November 2012.
Chunlaratthanaphorn S, Lertprasertsuke N, Srisawat U, Thuppia A, Ngamjariyawat A, Suwanlikhid N, Jaijoy K. Acute and sub chronic toxicity study of the water extract from root of Imperata cylindrica (Linn.) Raeusch. in rat. Songklanakarin J. Sci. Technol. March 2007; 29(Suppl. 1):141-155.
WHO. Traditional Medicine Growing Needs and Potential - WHO Policy Perspectives on Medicines. 2002;002, May, World Health Organization, Geneva, Switzerland. Mandy Horn. Available:https://blog.envigo.com/3-reasons-why-wistar-han-could-be-the-perfect-model-for-your-study
Gibbs RA, Weinstock GM, Metzker ML, Muzny DM, Sodergren EJ, Scherer S, Scott G, Steffen D, Worley KC, Burch PE, et al: Genome sequence of the Brown Norway rat yields insights into mammalian evolution. Nature. 2004;428:493-521. DOI:10.1038/nature02426. Article CAS PubMed Google Scholar
Zhao S, Shetty J, Hou L, Delcher A, Zhu B, Osoegawa K, de Jong P, Nierman WC, Strausberg, RL, Fraser CM. Human, mouse, and rat genome large-scale rearrangements: stability versus speciation. Genome Research. 2004;14(10A):1851–1860. DOI:https://doi.org/10.1101/gr.2663304
Johnston DE. Special considerations in interpreting liver function tests". Am Fam Physician 1999;59(8):2223–30. PMID 10221307.
McClatchey, Kenneth D. Clinical laboratory medicine. Lippincott Williams & Wilkins. 2002; 288–. ISBN 978-0-683-30751-1. Retrieved 5 July 2021.
Lala V, Goyal A, Bansal P, et al. Liver Function Tests. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2021 Jan. Available: https://www.ncbi.nlm.nih.gov/books/NBK482489/
Khadiga G. Adham, Atheer M. Asiri acute and chronic effects of aluminum smelter dust on hematology, metal bioaccumulation and oxidant-antioxidant status in rat. Agricultural Science Digest. 2021;41:218-224.
Mengel, Mark B, Schwiebert L. Peter. Family medicine: ambulatory care & prevention. McGraw-Hill Professional. 2005;268. ISBN 978-0-07-142322-9 Retrieved 5 August 2021.
Iverson C, Christiansen S, Flanagin A, et al. AMA manual of style: A guide for authors and editors. New York, NY: Oxford University Press; 2007. 10th ed.
Thomas JR, Nelson JK, Silverman SJ. Research methods in physical activity. Champaign, IL: Human Kinetics; 2005. 5th ed,
Altman DG. Practical statistics for medical research. New York, NY: Chapman & Hall. 1991;4–5.
Erhirhie EO, Emudainohwo JOT, Edafe EU. Effects of Vernonia amygdalina and Ocimum gratissimum combined leave extracts on blood glucose and biochemical parameters in alloxan induced diabetic rats. Continental J. Pharmacology and Toxicology Research. 2013;6(2):13-21.
Nayim P, Mbaveng AT, Ntyam AM, et al. A botanical from the antiproliferative Cameroonian spice, Imperata cylindrica is safe at lower doses, as demonstrated by oral acute and sub-chronic toxicity screenings. BMC Complement Med Ther. 2020;20:273. DOI:https://doi.org/10.1186/s12906-020-03064-6
Chunlaratthanaphorn S, Lertprasertsuke N, Srisawat U, Thuppia A, Ngamjariyawat A, Suwanlikhid N, Jaijoy K. Acute and sub chronic toxicity study of the water extract from root of Imperata cylindrica (Linn.) Raeusch. in rat. Songklanakarin J. Sci. Technol. March 2007; 29(Suppl. 1):141-155.
Oluwole Akinola, Michael Gabriel, Abdul-Azeez Suleiman, Felix Olorunsogbon. Treatment of alloxan-induced diabetic rats with metformin or glitazones is associated with amelioration of hyperglycaemia and neuroprotection. The Open Diabetes Journal. 2012;5:8-12.
Szkudelski T. The mechanism of alloxan and streptozotoxin action in B cells of the rat pancreas. Physiol. Res. 2001: 537-546.
Osasenaga Macdonald Ighodaro,Abiola Mohammed Adeosun, Oluseyi Adeboye Akinloye. Alloxan-induced diabetes, a common model for evaluating the glycemic-control potential of therapeutic compounds and plants extracts in experimental studies. Medicina. 2017;53(6):365-374.
Mbaka GO, Owolabi MA. Evaluation of haematinic activity and subchronic toxicity of Sphenocentrum jollyanum (menispermaceae) seed oil. European Journal of Medicinal Plants 2011;1(4):140-152.
Aitken RJ, Roman SD. Antioxidant systems and oxidative stress in the testes. Oxid Med Cell Longev. 2008;1(1):15-24.
Bhattacharyya D, Mukherjee R, Pandit S, Das N, Sur TK. Prevention of carbon tetrachloride induced hepatotoxicity in rats by Hmmoliv (a poly herbal formulation). Ind J Pharmacol. 2003;35:183-5.
Nyblom H, Björnsson E, Simrén M, Aldenborg F, Almer S, Olsson R. The AST/ALT ratio as an indicator of cirrhosis in patients with PBC. Liver Int. 2006;26 (7):840-5.
Dufour DR, Lott JA, Nolte FS, Gretch DR, Koff RS, Seeff LB. Diagnosis and monitoring of hepatic injury: II. Recommendations for use of laboratory tests in screening, diagnosis and monitoring. Clin Chem. 2000;46(12):2050-68.
McClatchey KD. Clinical laboratory medicine. 1st ed. Philadelphia: Lippincott Williams & Wilkins; 2002.
Gamal A. Mohamed et al. Chemical Composition and Hepato-protective activity of Imperata cylindrica. Beauv Research Article. 2009;5(17):28-36.
Saei H, Azarmi M, Dehghan G, Salmasi SZ, Zeinali S. Hepatoprotective effect of ethanolic extract of Lavandula officinalis L. in alloxan-induced diabetic rats. Thrita. 2016;5(2):e35306.
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