Open Access Original Research Article

Antioxidant Potentials of Aqueous Leaf Extract of Acalypha godseffiana on Hydrogen Peroxide-Induced Oxidative Stress in Wistar Rats

Samson Eruke Okoro, Stephen I. Omeodu, Favour Uzoma Iyke-David

Asian Journal of Research in Medical and Pharmaceutical Sciences, Page 1-14
DOI: 10.9734/ajrimps/2021/v10i430170

Aim: This study evaluated the efficacy of aqueous extract of Acalypha godseffiana leaves against H2O2-induced oxidative stress and organ damage in wistar rats by measuring hepatotoxic biomarkers, nephrotoxicity and status of the antioxidant defense system.

Study Design: Twenty-five rats, randomly divided into five groups, were used in this study. Fresh leaves of Acalypha godseffiana leaves were air-dried, ground into fine powder and used in the preparation of an aqueous extract.

Place and Duration of Study: Department of Biochemistry, and Department of Physiology, University of Port Harcourt, Nigeria between November 2020 and April 2021.

Methodology: Oxidative stress and toxicity was induced using 5ml of 6% H2O2. Treated rats received A. godseffiana aqueous leaf extract at various doses: 100, 200 and 400 mg/kg b.w. The rats were fasted for 24 h prior to sampling and then mildly anesthetized with chloroform. Biochemical assays and histological studies were conducted at days 14 and 28 respectively.

Results: There was significant (P<0.05) increase in plasma levels of AST in the H2O2 -treated group as compared with the negative control which recorded 105.00 ± 3.00 and 107.50 ± 7.50 (µ/L) at day 14 and 28 respectively. The H2O2 group showed significant (P<0.05) increases in plasma levels of K-, Na+, Urea, Cl- and HCO3- as compared with the negative control. A. godseffiana-treated rats showed significant (P<0.05) increase in serum levels of GSH, Catalase and SOD. Photomicrographs obtained showed histologically distorted liver and kidney tissues in the H2O2 group at days 14 and 28. Overall, the architecture of the liver was preserved by the administered aqueous leaf extract of A. godseffiana.

Conclusion: Data obtained from this study suggest that A. godseffiana leaves exhibit promising antioxidant and hepatoprotective potency, and consequently has ameliorative effects against H2O2-induced oxidative stress and organ damage in wistar rats.

Open Access Original Research Article

Effects of Medical Drugs According to Sweating Localization in Primary Hyperhidrosis

Bayrakçi Onur

Asian Journal of Research in Medical and Pharmaceutical Sciences, Page 22-25
DOI: 10.9734/ajrimps/2021/v10i430173

Background: Primary hyperhidrosis is excessive sweating localized to different parts of the body, mostly on the hands. It is exact cause  unknown, negatively affects the psychosocial structure of the person and a clinically important health problem.

Aims: In the study, it was aimed to examine the effects of drugs used on primary hyperhidrosis.

Study Design: The drugs used by the patients who applied to the thoracic surgery clinic with the complaint of sweating and their effects were analyzed retrospectively. Ersin Arslan Training and Research Hospital Thoracic Surgery Clinic between January 1, 2015, and September 30, 2021 (outcomes of seven years).

Methodology: A total of 120 patients(45 female, 75 male and age range 8-67) with sweating complaints were identified. Age, gender, sweating localization and drugs used were examined. Statistically, data were analyzed with 95% confidence interval(CI) and Chi Square test.

Results: A total of 120 patients were analyzed. 37.5% were female and 62.5% male.The mean age was 27.1±1.54 years.Patients were use 23.4% Aluminum hydrochloride cream),20.8% Bornaprine hydrochloride, 20% Hyoscine-N-butylbromide.According to sweating localizations;42.6% palmar only, 18.7% palmar and axilla, 18.7% palmar and craniofacial, 7.8% palmar and plantar, 5.9% diffuse, 4.5% cranial, 1.8% palmar and abdomen.It is more often between ages of 21-30 and in male.According to the complaints of patients with a history of using medical treatment; there were 34.5% partial response and 20.2% complete response, and no response to medical treatment in 45.3%.

Conclusion: In the study; according to the localization of sweating;Aluminum hydrochloride cream reduces sweating on the palmar, and Hyoscine-N-butylbromide reduces axillary and palmar sweating. Bornaprine hydrochloride reduces sweating on all localizations except craniofacial and abdomen, and is related with a complete response on palmar sweating.According to sweating localizations; although there are localizations where all three drugs used are effective, it has been concluded that these drugs used in primary hyperhidrosis do not respond fully at a high rate.

Open Access Original Research Article

Development & Validation of RP-HPLC Method for the Estimation of Doravirine in Bulk and Pharmaceutical Dosage Form

A. Suneetha, G. I. Priyadarshini, V. Mounika, G. Aparna

Asian Journal of Research in Medical and Pharmaceutical Sciences, Page 26-31
DOI: 10.9734/ajrimps/2021/v10i430174

A simple, accurate, rapid and precise isocratic reversed phase high-performance liquid chromatographic method has been developed and validated for determination of Doravirine in tablets. The chromatographic separation was carried out on Dionex C18 (250 x 4.6mm, 5µ) with a mixture of methanol: 0.05M potassium dihydrogen phosphate (40:60%v/v) as a mobile phase at a flow rate of 1.5 mL/min. UV detection was performed at 306 nm. The retention time was 5.24 min for Doravirine. Calibration plot was linear (r2=0.999) over the concentration range of 200-600 µg/mL. The method was validated for accuracy, precision, specificity, linearity, robustness, LOD and LOQ. The proposed method was successfully used for quantitative analysis of tablets. No interference from any component of pharmaceutical dosage form was observed. Validation studies revealed that method is specific, rapid, reliable, and reproducible. The high recovery and low relative standard deviation confirm the suitability of the method for routine determination of Doravirine in bulk and tablet dosage form.

Open Access Review Article

Placebo effect unboxed: A Review

Sharique Ahmad, Tanish Baqar, Shivani Singh, Saeeda Wasim, Shivangi Shukla, Huma Parveen

Asian Journal of Research in Medical and Pharmaceutical Sciences, Page 15-21
DOI: 10.9734/ajrimps/2021/v10i430172

As in our body, brain is the most powerful part and our mind could be excellent healing tool when any chance is provided. The idea that our brain can be one of the convincing parts in our body for the fake treatment is the real point this is called as placebo effect [1]. This effect refers to the impact of placebo on any one. However, treatments which are not active also were also demonstrated measurable and positive health response [2]. The ability of placebo effect is reviewed as psychological process [1]. In some cases, placebos can exert an influence powerful enough to mimic the effects of real medical treatments. This effect is more than positive thinking [3]. When this response occurs, many people have no idea they are responding to what is essentially a "sugar pill." Placebos are often utilized in medical research to help doctors and scientists discover and better perceive the physiological and mental effects new medications [2] For exactly understanding the placebo effect importance it is crucial to know more about how and why it works. This article explains how this effect is recognized in modern medicine and elements of placebo effect and suggests few conditions under which making utilization of therapeutic potential of this effect could be ethically acceptable, if not warranted.